Transverse section of skeletal muscle

Skeletal muscle is the easiest to start with. A skeletal muscle fiber is surrounded by a plasma membrane called the sarcolemma which contains sarcoplasm the cytoplasm of muscle cells.

This picture shows a transverse section through skeletal muscle.

Transverse section of skeletal muscle. Figure 3 - Skeletal Muscle transverse section. Bar is 50 microns In cross section skeletal muscle is identified by peripheral nuclei and large amounts of cytoplasm or small extracelluar space. The dark purple nuclei can be seen on the edges of the muscle fibres.

This picture shows a transverse section through skeletal muscle. If you cut a muscle transversely like this then you can see the roughly circular outline of the individual muscle fibres. The longitudinal section of a skeletal muscle will show the muscle fibres with their important regions which makes it very easy to understand the mechanism of muscle contraction and relaxation.

Transverse section is a cut made in a plane that is made across the body of an animal a plant an organ or a tissue. It is usually referred as the cut made between left and right. Note that sometimes the cross striations are not aligned all the way across the cell.

This is because different myofibrils may not be alignedIn transverse sections of skeletal muscle cells slide 58 even slide boxes observe thecylindrical shape of the cells fibers and the peripherally-located nuclei. Transverse section of skeletal muscle 40x. Shrinkage shows every cell distinctly.

Note two nuclei in a single cell marked by asterisks. All nuclei in cells are clearly in a peripheral location. In the transverse section skeletal muscle fibers are usually polygonal and their sarcoplasm often has a striated appearance as transversely sectioned myofibrils resolve as dots.

The packing density of muscle fibers varies from low in the outer muscles of the larynx to high in the muscle. When skeletal muscle is cut in cross-section you can appreciate all the cables pulling in the same direction. The only place youre likely to see interlacing bundles of skeletal muscle is in the tongue.

This is skeletal muscle cut transversely cross-wise. This makes it very easy to see the nuclei at the edges of the fibers. Eosin HE stained transverse muscle sections.

This SOP is written as a guide for those who wish to quantitate the amount of skeletal muscle necrosis as an indication of the extent of dystropathology and subsequent muscle regeneration in histological muscle sections from dystrophic mdx mice. Ticular muscle at the midline eg ventral abdomen linea alba epimysium G. On muscle fascia covering the surface of a muscle depending on the muscle it may be thin transparent or dense opaque.

Also perimysium ct. And endomysium ct. Within muscle fascicles Transverse section through a.

A section of the lip that shows transverse sections of skeletal muscle is examined by a group of first-year medical students shown in the image. Identify the structure indicated by the oval line. A Muscle fascicle B Muscle fiber C Myofibril D Myofilament E Sarcomere.

In transverse sections of skeletal muscle cells slide 58 observe the cylindrical shape of the cells fibers and the peripherally-located nuclei. Note also the the cytoplasm of the muscle cells has a stippled punctate appearance which is due to the bundling of thick and thin filaments into myofibrils as mentioned above. Skeletal muscle is mainly responsible for the movement of the skeleton but is also found in organs such as the globe of the eye and the tongue.

It is a voluntary muscle and therefore under conscious control. Skeletal muscle is specialized for rapid and forceful contraction of short duration. Muscle cells are much longer than they are broad and present different appearances in transverse and longitudinal sections.

It is important to recognise them both types of sections. Skeletal muscle is the easiest to start with. Skeletal muscle can be seen as a.

With longitudinal sections of skeletal muscle the nuclei may not always look like theyre in the periphery but with a transverse section its much easier to visualize. Its also easier to identify the endomysium which is the connective tissue that surrounds the polygonal muscle fibers. Of skeletal muscles is often assessed by histochemical techniques which enable the classification into different fiber types by combining the reactions in serial transverse muscle cross-sections.

A drawback is that a knowledgeable operator is required to combine and evaluate the reac-tions which is a time-consuming tedious and subjective. With cardiac muscle the heart becomes more effective at squeezing blood out of its chambers whereas skeletal muscle becomes more efficient at transmitting forces through tendonous attachments to bones 1. Skeletal muscle has two basic functions.

To contract to cause body movement and to provide stability for body posture. General anatomy of skeletal muscle its innervation and blood supply General anatomy of spinal nerve General terms of angiology and lymphology. Osteofascial spaces compartments transverse sections of body segments.

Attachments of skeletal muscles. Articular processes of C4-C6. Transverse processes of C7 and T1-T7.

Occipital bone between superior and inferior nuchal lines. Articular processes of C4-C7. Transverse processes of T1-T5.

Posterior margin of mastoid process. Posterior branch of spinal nerve. 15-11 Visibility of mitochondria formalin-fixed vs.

Frozen sections transverse sections of skeletal muscle. A Formalin-fixed paraffin-embedded sample. Mitochondria stain faintly and the myofibers lack detail when compared with B.

Broad tendon-like sheet of connective tissue that attaches a skeletal muscle to another skeletal muscle or to a bone depolarize to reduce the voltage difference between the inside and outside of a cells plasma membrane the sarcolemma for a muscle fiber making the inside less negative than at rest. Figure 1022 Muscle Fiber. A skeletal muscle fiber is surrounded by a plasma membrane called the sarcolemma which contains sarcoplasm the cytoplasm of muscle cells.

A muscle fiber is composed of many myofibrils which contain sarcomeres with light and. The columnar structure and surrounding skeletal muscles. Transverse sections at the level of the EAS and CSP.

Massons trichrome stain a immunostaining for skeletal muscle b and immunostaining for smooth muscle c. An elliptical crosssection of the columnar structure indicated by circles is observed anterior to the EAS.